Data sheet
| Assay time | 4 hours |
| Validity | 6 months |
| Specificity | No detectable cross-reactivity with any other cytokine. |
| Assay Range | 31.2--2000 pg/ml |
| Sensitivity | 2.0 pg/ml |
| Size | 96T |
| Storage | 2 - 8 ℃ |
| Application | serum, plasma, body fluids, tissue lysates or cell culture supernates. |
More info
Product name
Rat KIM1 ELISA Kit
Catalog No.
FM-E100227
Size
96T
Range
31.2pg/mL-2000pg/mL
Sensitivity
Less Than 2pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, plasma, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant Rat KIM1 standard: 10ng/tube-2.
ne 96-well plate precoated with anti- Rat KIM1 antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- Rat KIM1 antibody: 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
KIM1TIM-1 also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant Rat KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
Rat KIM1 ELISA Kit-1X96 Well Plate Image