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Rat Brain Natriuretic Peptide BNP ELISA Kit
BG-RAT10355
Rat Brain Natriuretic Peptide (Bnp) ELISA Kit
Data sheet
| Assay time | 4.5 hours |
| Assay Range | 12.5 - 800 pg/mL |
| Sensitivity | 1.2 pg/mL |
| Assay principle | The Rat BNP ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Rat BNP specific antibody has been pre-coated onto 96-well plate. Rat BNP present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Rat BNP detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Rat BNP captured on plate. |
| Sample type | serum/ plasma, cell culture supernatant and other suitable sample solution. |
More info
Different lot may vary, please follow the instruction coming with the kit.