UCHL1 Human

Data sheet

Formulation

The UCHL1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 2mM EDTA.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

UCHL1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 223 amino acids (1-223 a.a.) and having a molecular mass of 24.8 kDa (molecular weight on SDS-PAGE will appear higher).

Protein Background

UCHL1 (PGP9.5) belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. Protein Gene Product (PGP9.5) is a neuron specific protein, structurally and immunologically distinct from neuron spec

Expression host

Escherichia Coli.

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, EC 3.4.19.12, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, UCHL1, PGP9.5, PARK5.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA.

Biological Activity

Specific activity: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.

References

Title:Protein gene product 9.5 and ubiquitin are expressed in metabolically active epithelial cells of normal and pathologic human kidneyPublication:Nephrol Dial Transplant (2005) 20: 27142719 doi:10.1093/ndt/gfi124 Advance Access publication 27 September