Trypsin-2 Human

Data sheet

Formulation

The protein was lyophilized with 10mM Sodium Acetate buffer and 50mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Human Trypsin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

Recombinant Human Trypsin-2 is free from any animal and human sources.Recombinant Human Trypsin-2 expressed in E.Coli having a Mw of 24kDa is purified by standard chromatography techniques. Recombinant Human Trypsin-2 is free from foreign enzymes such as

Protein Background

Trypsin (EC3.4.21.4) is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site and no cleavage occurs if a p

Expression host

Escherichia Coli.

Reagent Appearance

Sterile Filtered lyophilized powder.

Stability

Recombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Unit Definition

One BAEE unit will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 at 25C (1cm light path). Trypsin digestion: suggested ratio (w:w) 1:20 to 1:1000 (Trypsin : Peptide).

Biological Activity

10,000 BAEE units/mg powder.