TIMP2 Human HEK

Data sheet

Formulation

The TIMP2 protein was lyophilized after extensive dialysis against PBS.

Solubility

It is recommended to reconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5) not less than 100ug/ml.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

TIMP2 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-2 (amino acids Cys27-Pro220) and is fused to a polyhistidine tag at the C-terminus.

Protein Background

TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role aga

Expression host

HEK293 Cells.

Synonyms

TIMP metallopeptidase inhibitor 2, CSC-21K, tissue inhibitor of metalloproteinase 2, TIMP-2, metalloproteinase inhibitor 2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a

Biological Activity

The IC50 of 3 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.