TGF b 1 Human, CHO

Data sheet

Formulation

The TGF b 1 Human 0.22 um filtered solution (0.24mg/ml) contains 20mM TRIS HCl buffer pH 7.2.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

TGFB1 Human Recombinant produced in CHO cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa.

Protein Background

Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are ve

Expression host

Chinese Hamster Ovary cells.

Synonyms

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

Biological Activity

The ED50 for this effect is typically 20-60ng/ml before activation and 0.1-1ng/ml after activation as measured by TGF-b1s ability to inhibit the mouse IL-4-dependent proliferation of HT-2 cells.Activation: Activated in vitro at pH below 3.5 and over 12.5.