TDG Human

Data sheet

Formulation

The TDG solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 0.4M Urea and 10% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

TDG Human Recombinant produced in E. coli is a single polypeptide chain containing 433 amino acids (1-410) and having a molecular mass of 48.4 kDa.TDG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Protein Background

Thymine-DNA glycosylase (TDG) is a member of the TDG/mug DNA glycosylase family. TDG is a nuclear protein that fixes G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thym

Expression host

E.coli.

Synonyms

Thymine-DNA Glycosylase, G/T Mismatch-Specific Thymine DNA Glycosylase, EC 3.2.2.29.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FR