sRANK Receptor Human

Data sheet

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20mM Tris-HCl, pH 8.0 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized sRANK Receptor in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

sRANK Receptor Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a molecular mass of 19.1kDa. The sRANK Receptor is purified by proprietary chromatographic techniques.

Protein Background

sRANK Receptor is a part of of the TNF superfamily of ligands and receptors which participates in the regulation of specific immunity and bone turnover. sRANK Receptor was originally acknowledged as a dendritic-cell-membrane protein, which by interacting

Expression host

Escherichia Coli.

Synonyms

Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, ODFR, Receptor activator of NF-KB, FEO, OFE, ODFR, OSTS, PDB2, RANK, CD265, OPTB7, TRANCER, LOH18CR1.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized sRANK Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANK Receptor should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is re

Amino acid sequence

QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK.

Biological Activity

The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B (NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Lig

Fomrulation

Title: Tissue factor-Akt signaling triggers microvessel formationPublication: DOI: 10.1111/j.1538-7836.2012.04848.x, 4 SEP 2012Link: https://http://onlinelibrary.wiley.com/doi/10.1111/j.1538-7836.2012.04848.x/full