SKA1 Human

Data sheet

Formulation

The SKA1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.1M NaCl.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

SKA1 Human Recombinant produced in E. coli is a single polypeptide chain containing 278 amino acids (1-255) and having a molecular mass of 31.9 kDa.SKA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques

Protein Background

Spindle And Kinetochore Associated Complex Subunit 1 (SKA1) is a part of the kinetochore-microtubule interface which associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule

Expression host

Escherichia Coli.

Synonyms

Spindle and kinetochore-associated protein 1, SKA1, C18orf24, Spindle And Kinetochore Associated Complex Subunit 1.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLT YNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RY