SERPINA4 Human

Data sheet

Formulation

SERPINA4 was filtered (0.4um) and lyophilized from 0.5mg/ml solution in phosphate buffered saline pH 7.4 and 5% (w/v) Trehalose.

Solubility

It is recommended to add 200ul deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. SERPINA4 is not sterile! Please filter the product by an appropriate sterile filter before us

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

SERPINA4 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Gln21-Pro427) containing a total of 417 amino acids, having a calculated molecular mass of 47.7kDa and fused to a 10 aa His tag at C-Terminus.

Protein Background

Kallistatin (SERPINA4) inhibits human amidolytic and kininogenase activities of tissue kallikrein. This inhibition is attained by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and production of a small C-ter

Expression host

HEK 293.

Synonyms

Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin) Member 4, PI4, KST, Serine (Or Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4, Peptidase Inhibitor 4, Kallikrein Inhibitor, Serpin A4, PI-4,

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

QLHVEHDGES CSNSSHQQIL ETGEGSPSLK IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ EH