S100B Human, His

Data sheet

Formulation

S100B (1mg/ml) is supplied in 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

S100B Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 112 amino acids fragment (1-92) with a 20 amino acids N-terminal His tag and having a total molecular mass of 12.8kDa. The S100B is purified by propriet

Protein Background

S100b is a member of the S100 family of proteins which are a family of EF-hand calcium binding proteins that exist mostly as dimers of the 20 currently identified individual S100 monomers. The S100B homodimer is expressed in cells of the central nervous s

Expression host

Escherichia Coli.

Synonyms

Protein S100-B, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, S100B, NEF, S100, S100beta.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

References

Title: Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells Publication: Molecular human reproduction 16.3 (2010): 188-199. Link: http://molehr.oxfordjournals.org/content/16/3/188.full

Immunological tests

Binds IgG-type human auto-antibodies.

Shipping Conditions

Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test.