PTH (7-34) Human

Data sheet

Formulation

The protein (1 mg/ml) was lyophilized after extensive dialyses against 20mM PBS pH-7.0 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized PTH in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Parathyroid Hormone Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 28 amino acids and having a molecular mass of 3380 Dalton. The PTH is purified by proprietary chromatographic techniques.

Protein Background

Polypeptide hormones secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote ren

Expression host

Escherichia Coli.

Synonyms

PTH/PTHrP receptor antagonist, PTHrP analog, PTHR, MGC138426, MGC138452.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add

Amino acid sequence

LMHNLGKHLN SMERVEWLRK KLQDVHNF.

Biological Activity

The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 10,000 Units/mg.