PTH (1-84) N15 Human

Data sheet

Formulation

PTH (1-84) N15 protein was lyophilized from a 0.2um filtered concentrated solution in 1xPBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Parathormone in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

PTH (1-84) N15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 84 amino acids and having a molecular mass of 9550 Dalton labeled by the stable isotope N15.The PTH (1-84) N15 is purified by proprietary chroma

Protein Background

Parathyroid hormone (PTH), or parathormone, is secreted by the parathyroid glands as a polypeptide containing 84 amino acids. It acts to increase the concentration of calciumin the blood, whereas calcitonin (a hormone produced by the parafollicular cells

Expression host

Escherichia Coli.

Synonyms

Parathyrin, PTH, Parathormone.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add

Amino acid sequence

SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ.

Biological Activity

The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 9,000 Units/mg.

Protein concentration

Lateral flow immunoassay.

link

Purified monoclonal IgG by protein A chromatography.