PTH (1-84) Human

Data sheet

Formulation

Each 1.3mg of lyophilized protein contains 50ug Mannitol and 20mM PB, pH 7.0.

Solubility

It is recommended to reconstitute the lyophilized Parathormone in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Parathyroid Hormone 1-84 (full length) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 84 amino acids, having an MW of ~10kDa.The PTH is purified by proprietary chromatographic techniques.

Protein Background

Parathyroid hormone (PTH), or parathormone, is secreted by the parathyroid glands as a polypeptide containing 84 amino acids. It acts to increase the concentration of calciumin the blood, whereas calcitonin (a hormone produced by the parafollicular cells

Expression host

Escherichia Coli.

Synonyms

Parathyrin, PTH, Parathormone.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add

Amino acid sequence

SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ.

Biological Activity

The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 9000 Units/mg.