PKACa2- RIa2

Data sheet

Formulation

PKA holoenzyme type-I alpha is supplied in 50% glycerol.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Inactive holoenzyme consisting of one dimeric regulatory subunit type I alpha and two monomeric catalytic subunits (cAMP-free). Protein Kinase A Recombinant is purified by proprietary chromatographic techniques.

Protein Background

The protein kinase A holoenzyme is a heterotetramer composed of two types of subunits: Catalytic and Regulatory. The Catalyticsubunit contains the enzyme's active site. It also contains a domain that binds ATP and a domain that binds the regulatory subuni

Expression host

Escherichia Coli.

Synonyms

Protein Kinase A holoenzyme type I alpha, PKACa2- RIa2.

Reagent Appearance

Sterile Filtered clear solution.

Stability

PKA should be stored at 4°C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20°C. Avoid multiple freeze-thaw cycles.

Biological Activity

Holoenzyme can be activated by adding the second messenger cAMP (Activation constant about 100nM) releasing two monomeric catalytic subunits.