PDLIM1 Human

Data sheet

Formulation

The PDLIM1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT, 2mM EDTA and 10% glycerol.

Purity

Greater than 85% as determined by SDS-PAGE.

Description

PDLIM1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-329) and having a molecular mass of 38.7 kDa. PDLIM1 is fused to a 25 amino acid His-tag at N-terminus.

Protein Background

PDLIM1, a cytoplasmic protein linked to the cytoskeleton, belongs to the enigma protein family. PDLIM1 holds two protein interacting domains - PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. PDLIM1 enables bring

Expression host

Escherichia Coli.

Synonyms

PDZ And LIM Domain Protein 1, Carboxyl Terminal LIM Domain Protein 1, Epididymis Secretory Protein Li 112m, C-Terminal LIM Domain Protein 1, LIM Domain Protein CLP-36, hCLIM1, HEL-S-112, CLP-36, Elfin.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMTTQQ IDLQGPGPWG FRLVGGKDFE QPLAISRVTP GSKAALANLC IGDVITAIDG ENTSNMTHLE AQNRIKGCTD NLTLTVARSE HKVWSPLVTE EGKRHPYKMN LASEPQEVLH IGSAHNRSAM PFTASPASST TARVITNQYN NPAGLYSSEN ISNFNNALES KTAASGVEAN SRPLDHAQPP SSLVIDKESE VYKMLQEKQE LN