PDGF BB Human, HEK

Data sheet

Formulation

The PDGF-BB protein was lyophilized from 1mg/ml in Acetonitrile and TFA.

Solubility

It is recommended to reconstitute the lyophilized PDGF-BB in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

PDGF-BB Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight of 25kDa due to glycosylation.The PDGF-BB is purified by proprietary chromatographic techniques.

Protein Background

PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer

Expression host

HEK.

Synonyms

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin.

Stability

Lyophilized PDGF-BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-BB should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to ad

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

Assay # 1: The biological activity was measured by its binding ability in a functional ELISA, the ED50 is typically 0.02-0.6 ug/ml.Assay # 2: The biological activity was determined by the dose-dependent stimulation of the proliferation of mouse 3T3 cells,