NSMCE1 Human

Data sheet

Formulation

The NSMCE1 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

NSMCE1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 289 amino acids (1-266) and having a molecular mass of 33.0kDa.NSMCE1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chroma

Protein Background

NSMCE1 is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand brea

Expression host

Escherichia Coli.

Synonyms

Non-SMC Element 1 Homolog (S. Cerevisiae), NSE1, Non-Structural Maintenance of Chromosomes Element 1 Homolog, Non-SMC Element 1 Homolog, EC 6.3.2.-.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMQGSTRR MGVMTDVHRR FLQLLMTHGV LEEWDVKRLQ THCYKVHDRN ATVDKLEDFI NNINSVLESL YIEIKRGVTE DDGRPIYALV NLATTSISKM ATDFAENELD LFRKALELII DSETGFASST NILNLVDQLK GKKMRKKEAE QVLQKFVQNK WLIEKEGEFT LHGRAILEME QYIRETYPDA VKICNICHSL LIQGQSCETC GI