NRN1 Human, His

Data sheet

Formulation

NRN1 was filtered (0.4 um) and lyophilized in 20mM Tris buffer, 50mM NaCl, pH 8.0, 1% (w/v) Sucrose and 4% (w/v) Mannitol.

Solubility

It is recommended to add 200ul of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. NRN1 is not sterile! Please filter the product by an appropriate sterile filter before usi

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Neuritin-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ala28-Gly116) containing 99 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 11.02kDa.

Protein Background

Neuritin (NRN1) is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3 and other neural stimulators. NRN1 is expressed principally in postmitotic-differentiating neurons of the developing nervous syste

Expression host

Escherichia Coli.

Synonyms

Neuritin 1, NRN1, NRN, dJ380B8.2, Neuritin.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.

Amino acid sequence

MKHHHHHHASAGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGNG.

PCR Protocol

200mM Tris-HCl (pH 8.8 at 25°C), 100mM (NH4)2SO4, 100mM KCl, 1% Triton X-100, 1mg/ml BSA, 20mM MgSO4.

Inhibitory Activity

Add the following components to amplify 1kb DNA template: 0.2ul Pfu-DNA Polymerase.4ul 2.5mM dNTPs.5ul 10x buffer with MgSO4. 1ul Primers mix (10uM each).1ul Template.38ul ddH2O. Amplify using the following cycling parameters: Heat Soak: 1 cycle at 94°C/4