NOB1 Human

Data sheet

Formulation

The NOB1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

NOB1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 435 amino acids (1-412) and having a molecular mass of 49.1kDa.NOB1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatogr

Protein Background

Nob1 takes part in pre-rRNA processing and cleaves in a late cytoplasmic processing step a 20S rRNA intermediate at cleavage site D to create the mature 18S rRNA. In yeast, more than 200 protein and RNA cofactors are essential for ribosome assembly, and t

Expression host

Escherichia Coli.

Synonyms

NIN1/RPN12 Binding Protein 1 Homolog, PSMD8BP1, PSMD8 Binding Protein 1, Nin One Binding Protein, Phosphorylation Regulatory Protein HP-10, Protein ART-4, RNA-Binding Protein NOB1, Adenocarcinoma Antigen Recognized By T Lymphocytes 4, NOB1P, MST158.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMAPVEHV VADAGAFLRH AALQDIGKNI YTIREVVTEI RDKATRRRLA VLPYELRFKE PLPEYVRLVT EFSKKTGDYP SLSATDIQVL ALTYQLEAEF VGVSHLKQEP QKVKVSSSIQ HPETPLHISG FHLPYKPKPP QETEKGHSAC EPENLEFSSF MFWRNPLPNI DHELQELLID RGEDVPSEEE EEEENGFEDR KDDSDDDGGG WI