NCS1 213 a.a. Human

Data sheet

Formulation

NCS1 213 a.a. protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

NCS1 213 a.a. Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-190 a.a) and having a molecular mass of 24.3kDa. NCS1 213 a.a. is fused to a 23 amino acid His-tag at N-terminus & purified b

Protein Background

NCS1 is part of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed primarily in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. NCS1

Expression host

Escherichia Coli.

Synonyms

Neuronal Calcium Sensor 1, Frequenin-Like Ubiquitous Protein, Frequenin-Like Protein, Frequenin Homolog, FREQ, FLUP, Frequenin (Drosophila) Homolog, Frequenin Homolog (Drosophila), FREQ, NCS-1, Neuronal calcium sensor 1.

Reagent Appearance

Sterile Filtered clear colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMGKSNSK LKPEVVEELT RKTYFTEKEV QQWYKGFIKD CPSGQLDAAG FQKIYKQFFP FGDPTKFATF VFNVFDENKD GRIEFSEFIQ ALSVTSRGTL DEKLRWAFKL YDLDNDGYIT RNEMLDIVDA IYQMVGNTVE LPEEENTPEK RVDRIFAMMD KNADGKLTLQ EFQEGSKADP SIVQALSLYD GLV.

Immunological Activity

Thimerosal and beta propiolactone treatment.This product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are req

Reference

Serological studies of Parainfluenza virus, immunogen for antibody production.