MYL9 Mouse

Data sheet

Formulation

MYL9 protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Inactivation

Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of

Description

MYL9 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-172 a.a) and having a molecular mass of 22.4kDa.MYL9 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chroma

Protein Background

MYL9 is one of the numerous regulatory myosin light chains. Myosin which is a structural component of the muscle consists of 2 heavy chains and 4 light chains. MYL9 is a myosin light chain regulates muscle contraction by modulating the ATPase activity of

Expression host

Escherichia Coli.

Synonyms

Myosin regulatory light polypeptide 9, Myosin regulatory light chain 2, Myosin regulatory light chain 9, Myl9, Myrl2.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSSKRA KAKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DEYLEGMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEASG FIHEDHLREL LTTMGDRFTD EEVDEMYREA PIDKKGNFNY VEFTRILKHG AKDKDD