MMP-2 Human, HEK

Data sheet

Formulation

The MMP-2 is supplied as a 0.2um filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

MMP-2 Human Recombinant produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having an Mw of 71kDa. MMP-2 is purified by proprietary chromatographic techniques.

Protein Background

Matrix metalloproteinase-2 (MMP-2) is a type IV collagenase, which is involved in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. MMP-2 contains a number of distinct domains: a prodomain that is cleaved upon a

Expression host

HEK293 cells.

Synonyms

72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II.

Reagent Appearance

The MMP-2 is supplied as a sterile Filtered colorless solution.

Stability

Store MMP-2 at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.

Biological Activity

The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is > 1,000 pmoles/min/ug.Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylm

References

Title:MMP-2 regulates human platelet activation by interacting withintegrin aIIbb 3.Publication:To cite this article: Choi W-S, Jeon O-H, Kim H-H, Kim D-S. MMP-2 regulates human platelet activation by interacting with integrin aIIbb3.J Thromb Haemost 2008