MMP 7 Human, Active

Data sheet

Formulation

The protein contains the following additives 10mM HEPES (pH 7.4), 5mM CaCl2 and 150mM NaCl.

Description

Matrix Metalloproteinase-7 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13 kDa.The MMP-7 is purified by proprietary chromatographic techniques.

Protein Background

Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin fam

Expression host

Escherichia Coli.

Synonyms

Matrilysin, EC 3.4.24.23, Pump-1 protease, Uterine metalloproteinase, Matrix metalloproteinase-7, MMP-7, Matrin, MPSL1, PUMP-1, MMP7.

Reagent Appearance

Sterile clear liquid solution.

Stability

Matrilysin although stable at 4°C for 3 weeks, should be stored below -18°C. Please prevent freeze-thaw cycles.

Unit Definition

One unit is defined as the digestion of 1 ug Azocoll/min at 37°C.

Biological Activity

The specific activity was found to be 6,200 U/mg.

References

Title: Interaction of Periosteal Explants with Articular Chondrocytes AltersExpression Pro?le of Matrix MetalloproteinasesPublication: Journal of Orthopaedic Research 28.12 (2010): 1576-1585.Link:http://onlinelibrary.wiley.com/doi/10.1002/jor.21154/epdf