LIF Human, HEK

Data sheet

Formulation

The LIF protein was lyophilized from 1mg/ml in PBS pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized LIF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Leukemia Inhibitory Factor Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 19.9kDa due to glycosylation.The LIF is purified by proprietary chromatographic techniques.

Protein Background

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentia

Expression host

HEK.

Synonyms

CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.

Stability

Lyophilized LIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LIF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carr

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The biological activity was determined by its ability to stimulate the proliferation of Human TF-1 cells, the ED50 is typically less than 0.1ng/ml.