LGALS9 Mouse

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The LGALS9 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

LGALS9 mouse Recombinant produced E. coli is a single polypeptide chain containing 345 amino acids (1-322) and having a molecular mass of 38kDa.LGALS9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Protein Background

LGLAS9 binds galactosides and has high affinity for the Forssman pentasaccharide. LGLAS9 participates in thymocyte-epithelial interactions relevant to the biology of the thymus and Inhibits cell proliferation. LGLAS9 is a ligand for HAVCR2/TIM3. LGLAS9 In

Expression host

Escherichia Coli.

Synonyms

Galectin-9, Gal-9, Lgals9, Lgals5, LGALS35, AA407335, AI194909, AI265545.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMALFSAQ SPYINPIIPF TGPIQGGLQE GLQVTLQGTT KSFAQRFVVN FQNSFNGNDI AFHFNPRFEE GGYVVCNTKQ NGQWGPEERK MQMPFQKGMP FELCFLVQRS EFKVMVNKKF FVQYQHRVPY HLVDTIAVSG CLKLSFITFQ TQNFRPAHQA PMAQTTIHMV HSTPGQMFST PGIPPVVYPT PAYTIPFYTP IPNGLYPSKS IM

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Cytotoxic and staining antibody. For staining, use 10ml/1,000,000 cells. Titer for cytotoxicity should be determined by the investigator.

Immunological function

This antibody is also available conjugated to biotin and FITC.

Product Description

Recognizes most MHC I-E class II haplotypes Reacts with H-2k, H-2d, H-2p and H-2r.