KRT20 Antibody

Data sheet

Clone

PAT10D8AT.

Applications

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:200.

Protein Background

KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic protein

Synonyms

Keratin type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20, CD20, CK20, KRT21, MGC35423.

Reagent Appearance

Sterile filtered colorless solution.

Type

Mouse Anti Human Monoclonal.

Immunogen

Anti-human KRT20 mAb, clone PAT10D8AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT20 protein 1-424 amino acids purified from E. coli.

Ig Subclass

Mouse IgG2a heavy chain and k light chain.

Purification Method

KRT20 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Protein formulation

1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.

Storage Procedures

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

Stability / Shelf Life

12 months at -20°C. 1 month at 4°C.