IL1A Human, HEK

Data sheet

Formulation

The IL-1A protein was lyophilized from a 0.2um filtered solution of PBS pH 7.4 with 10% trehalose as protectant.

Solubility

It is recommended to reconstitute the lyophilized IL1A in deionized water to a stock solution of 0.5mg/ml.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Description

Interleukin-1 alpha Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 18kDa due to glycosylation.The IL1A is purified by proprietary chromatographic techniques.

Protein Background

IL-1 alpha is produced by activated macrophages, stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1A proteins are involved in the inflammatory response, being identi

Expression host

HEK.

Synonyms

Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.

Stability

Lyophilized IL-1A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a c

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

Assay # 1: The biological activity was measured by its binding ability in a functional ELISA, the ED50 is typically 0.5-5 ug/ml. Assay # 2: The biological activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells,

Protein concentration

L-Selectin can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.