IL 4 Human, Yeast

Data sheet

Formulation

The protein was lyophilized from 0.2um filtered solution in 20mM sodium phosphate buffer pH 6.0 in absence of any carrier protein.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98% as determined by SDS-PAGE.

Description

Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.The IL-4 is purified by proprietary chromatographic techniques.

Protein Background

IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transduce

Expression host

Pichia pastoris.

Synonyms

BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin-4 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycle

Biological Activity

The biological activity is determined by measuring the dose-dependent proliferation of human TF1 cells and CD23 expression. A concentration range of 0.110.0 ng/ml is effective for most in vitro applications. ED50 = 0.050.4ng/ml.