HMGB3 Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The HMGB3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol.

Purity

Greater than 85% as determined by SDS-PAGE.

Description

HMGB3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (1-180) and having a molecular mass of 22.8kDa.HMGB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromato

Protein Background

HMGB3 is a member of the high mobility group (HMG) protein superfamily. HMGB3 holds DNA-binding HMG box domains, as do HMG1 and HMG2, and like the rest of the HMG protein superfamily members has a vital part in DNA replication, transcription and nucleosom

Expression host

Escherichia Coli.

Synonyms

High Mobility Group Box 3, High-Mobility Group (Nonhistone Chromosomal) Protein 4, Non-Histone Chromosomal Protein, High Mobility Group Protein B3, High Mobility Group Protein 4, HMG-2a, HMG-4.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMAKGDPK KPKGKMSAYA FFVQTCREEH KKKNPEVPVN FAEFSKKCSE RWKTMSGKEK SKFDEMAKAD KVRYDREMKD YGPAKGGKKK KDPNAPKRPP SGFFLFCSEF RPKIKSTNPG ISIGDVAKKL GEMWNNLNDS EKQPYITKAA KLKEKYEKDV ADYKSKGKFD GAKGPAKVAR KKV.

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:5,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).