HMGA2 Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

HMGA2 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT and 250mM Imidazole.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

HMGA2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 117 amino acids (1-109 a.a) and having a molecular mass of 12.8kDa.HMGA2 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chro

Protein Background

High Mobility Group AT-Hook 2 (HMGA2) is a member of the non-histone chromosomal high mobility group (HMG) family. HMG proteins perform as architectural factors and are necessary components of the enhancesome. This protein contains structural DNA-binding

Expression host

Escherichia Coli.

Synonyms

High Mobility Group AT-Hook 2, HMGIC, High-Mobility Group (Nonhistone Chromosomal) Protein Isoform I-C, High Mobility Group AT-Hook Protein, BABL, HMGI-C, LIPO, STQTL9, High Mobility Group Protein HMGI-C, HMGA2.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MSARGEGAGQ PSTSAQGQPA APAPQKRGRG RPRKQQQEPT GEPSPKRPRG RPKGSKNKSP SKAAQKKAEA TGEKRPRGRP RKWPQQVVQK KPAQEETEET SSQESAEEDL EHHHHHH

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:5,000 dilution will yield 0.1 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).