HEV ORF2 (403-461 a.a.)

Data sheet

Storage

HEV ORF2 protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Formulation

20mM Tris-HCl pH-8, 8M urea and 10mM B-ME.

Purity

HEV ORF2 Protein is >95% pure as determined by 10% PAGE (coomassie staining).

Applications

HEV ORF2 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.

Description

The e.coli derived HEV protein is fused with beta-galactosidase at the N-Terminus and contains the HEV immunodominant regions from ORF2 403-461 a.a.

Protein Background

Hepatitis E virus (HEV), the major etiologic agent of enterically transmitted non-A, non-B hepatitis worldwide, is a spherical, non-enveloped, single stranded RNA virus that is approximately 32 to 34 nm in diameter. HEV belongs to a genus of HEV-like viru

Purification Method

HEV ORF2 was purified by proprietary chromatographic technique.

Advantages

Blood samples from each donor have been tested and found negative for HBsAg, anti-HCV, HIV Ag/Ab, and syphilis.