Gly m 5.0101

Data sheet

Formulation

Gly m 5.0101 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

Recombinant Alpha Subunit of Beta Conglycinin produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 70,586 Dalton. Gly m 5.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic t

Protein Background

Alpha Subunit of Beta Conglycinin (Gly m 5.0101) causes an allergic reaction in humans.

Expression host

Sf9 insect cells.

Synonyms

Alpha subunit of beta conglycinin, Gly m 5.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgE type human antibodies. 2. Immunodot test with positive/negative sera panels.

Molar extinction coefficient

35090; A280(1mg/ml)=0.497