Gliadin Gamma 18.6kDa Wheat

Data sheet

Formulation

Gliadin is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl, and 20% glycerol.

Purity

Greater than 94% as determined by SDS-PAGE.

Applications

Western blot with monoclonal anti-hexa-His-tag antibody.

Description

Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform, having a molecular mass of 18,616 Dalton (high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight an

Protein Background

Wheat Gliadin and related gluten components from barley, rye and possibly oats can cause an abnormal immune response called Celiac disease which is a chronic gastrointestinal disorder. Celiac disease characteristics are flattening of the jejunal mucosa an

Expression host

Escherichia Coli.

Reagent Appearance

Sterile Filtered clear solution.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Coating concentration

0.15-0.5 ug/ml (depending on the type of ELISA plate and coating buffer).

Reference

Western-Blot 0.1ug -1ug per strip, ELISA 1ug/Well.