GAD2 Human

Data sheet

Formulation

GAD2 protein solution is supplied in 16mM HEPES buffer pH-7.5, 250mM NaCl, 0.016mM Pyridoxal-5-Phosphate, 0.16% Triton x-100 and 20% glycerol.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Recombinant Human GAD2 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66,234 Dalton. GAD2 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Protein Background

Glutamate Decarboxylase 2 (GAD2) is one of several forms of glutamic acid decarboxylase, identified as a main autoantigen in insulin-dependent diabetes. The GAD2 enzyme is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutami

Expression host

Sf9 insect cells.

Synonyms

Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kDa), Glutamate Decarboxylase 65 KDa Isoform, 65 KDa Glutamic Acid Decarboxylase, EC 4.1.1.15, GAD-65, GAD65, Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kD), Glutamate Decarboxylase-

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels).