FNDC5 Human, Yeast

Data sheet

Formulation

The FNDC5 was lyophilized from 0.45 um filtered solution in PBS.

Solubility

It is recommended to reconstitute the lyophilized Fibronectin Type III Domain Containing 5 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by SDS-PAGE.

Description

Fibronectin Type III Domain Containing 5 Human Recombinant produced in yeast is a glycosylated, polypeptide chain containing 110 amino acids and having a molecular mass of 20-25 kDa.FNDC5 is purified by proprietary chromatographic techniques.

Protein Background

Fibronectin Type III Domain Containing 5 (Irisin) is a newly discovered hormone, secreted into circulation by muscle, which acts on white adipocytes and promotes WAT browning. r-Irisin can reduce body weight and cause induction of brown adipocyte in vivo,

Expression host

Yeast.

Synonyms

Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin, FRCP2, FNDC5.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Fibronectin Type III Domain Containing 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FNDC5 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent

Amino acid sequence

S_P__S__A__P__V__N__V__T__V__R__H__L__K__A__N__S__A__V__V__S_W__D__V__L__E__D__E__V__V__I__G__F__A__I__S__Q__Q__K__K__D _V__R__M__L__R__F__I__Q__E__V__N__T__T__T__R__S__C__A__L__W__ D__L__E__E__D__T__E__Y__I__V__H__V__Q__A__I__S__I__Q__G__Q_S__P__A__S__E_

Biological Activity

The biological activity of recombinant human irisin was measured by the ability to induce UCP-1 expression of adipocytes. The specific activity is 10ng/ml.