FGF 2 Human (147 a.a.)

Data sheet

Formulation

The bFGF was lyophilized from a sterile filtered solution containing 10mM sodium phosphate, 75mM sodium chloride, pH 7.5.

Solubility

It is recommended to reconstitute the lyophilized FGF-B in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.5kDa. The FGF2 is purified by proprietary chromatographic techniques.

Protein Background

FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis

Expression host

Escherichia Coli.

Synonyms

Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGFb should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add

Amino acid sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS.

Biological Activity

The ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is 0.018-0.027ng/ml corresponding to a specific activity of 5.6x107 units/mg.