EPO a Human, His

Data sheet

Formulation

EPO a protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Purity

Greater than 80% as determined by SDS-PAGE.

Description

EPO a Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids (28-193a.a.) and having a molecular mass of 20.9kDa.EPO a is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chr

Protein Background

EPO a is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. EPO a is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin s

Expression host

Escherichia Coli.

Synonyms

Erythropoietin, EP, INN=Epoetin, EPO a, Erythropoietin-alpha, EPO.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSAPPRLIC DSRVLERYLL EAKEAENITT GCAEHCSLNE NITVPDTKVN FYAWKRMEVG QQAVEVWQGL ALLSEAVLRG QALLVNSSQP WEPLQLHVDK AVSGLRSLTT LLRALRAQKE AISPPDAASA APLRTITADT FRKLFRVYSN FLRGKLKLYT GEACRTGDR.