Ebola Zaire VP40

Data sheet

Formulation

Ebola Zaire VP40 protein solution is supplied in Phosphate buffer, 25mM arginine and 0.02% sodium azide.

Purity

Greater than 95% as determined by 12% PAGE (Coomassie staining).

Applications

Immunoassay.

Description

Recombinant Ebola Zaire VP40 is a full length of Zaire Ebola VP40 containing 325 amino acids produced in E.coli, having an Mw of 35kDa, and fused to a 6xHis tag at its C-terminus.Ebola Zaire VP40 is purified by a proprietary chromatographic technique.

Protein Background

Ebolavirus (EVD) belongs to the Filoviridae family of proteins which is comprised of a single-strand, non-infectious RNA genome. EVD genome is about 19,000 base pairs long and covers 7 genes in the order 3'-UTR-NP-VP35-VP40-GP-VP30-VP24-L-5'-UTR. There ar

Expression host

Escherichia Coli.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Protein content

50mM Tris-Hcl, pH 8.0 at 25C, 500mM NaCl, 1mM CaCl2 and 50% glycerol.

Rapid Test Assay

50mM Tris-HCl or sodium phosphate (pH 8.0) at 25°C with or without CaCl2. The enzyme is active at a pH range of 6.0-9.0.