CRYM Human

Data sheet

Formulation

CRYM was filtered (0.4um) and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl, pH 7.5.

Solubility

It is recommended to add 200ul of deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. CRYM is not sterile! Please filter the product by an appropriate sterile filter before usin

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

CRYM Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ser2-Lys314) containing 322 amino acids including a 9 aa His tag at N-terminus. The total calculated molecular mass is 34.8kDa.

Protein Background

Crystallin, Mu (CRYM) is a taxon-specific crystallin protein which binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. CRYM doesnt perform a structural role in lens tissue; instead CRYM binds thyroid hormone for possible regula

Expression host

Escherichia Coli.

Synonyms

Crystallin Mu, Thiomorpholine-Carboxylate Dehydrogenase, THBP, NADP-Regulated Thyroid-Hormone Binding Protein, NADP-Regulated Thyroid-Hormone-Binding Protein, Mu-Crystallin Homolog, EC 1.5.1.25, DFNA40, CRYM.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MKHHHHHHAS RVPAFLSAAE VEEHLRSSSL LIPPLETALA NFSSGPEGGV MQPVRTVVPV TKHRGYLGVM PAYSAAEDAL TTKLVTFYED RGITSVVPSH QATVLLFEPS NGTLLAVMDG NVITAKRTAA VSAIATKFLK PPSSEVLCIL GAGVQAYSHY EIFTEQFSFK EVRIWNRTKE NAEKFADTVQ GEVRVCSSVQ EAVAGADVII TVTLATEPIL FGEWVKPGAH IN