CKB Human, Pichia

Data sheet

Formulation

CKBB Human contains 10Mm Bis-Tris-HCl pH-6.0, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

CKBB Human Recombinant produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to BB Isoenzyme in

Protein Background

Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in oth

Expression host

Pichia Pastoris.

Synonyms

Creatine kinase B-type, EC 2.7.3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI.

Reagent Appearance

Sterile Filtered colourless liquid formulation.

Stability

CKBB should be stored below -18°C. Please prevent freeze-thaw cycles.

Biological Activity

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 umole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml.