CHI3L2 Human

Data sheet

Formulation

CHI3L2 was filtered (0.4 um) and lyophilized from 0.5mg/ml solution in 30mM acetate buffer, pH 4.

Solubility

It is recommended to add 0.1M acetate buffer pH 4.0 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer t

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

CHI3L2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Tyr27-Leu390) containing 374 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 42.1kDa.

Protein Background

Chitinase 3-Like 2 (CHI3L2) is similar to bacterial chitinases but lacks chitinase activity. CHI3L2 protein is secreted and is involved in cartilage biogenesis. CHI3L2 is a lectin, which binds chitooligosaccharides and other glycans with high affinity, bu

Expression host

Escherichia Coli.

Synonyms

Chitinase 3-Like 2, Chondrocyte Protein 39, YKL-39, YKL39, Chitinase-3-Like Protein 2, CHIL2, CHI3L2.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MKHHHHHHASYKLVCYFTNW SQDRQEPGKF TPENIDPFLC SHLIYSFASI ENNKVIIKDK SEVMLYQTIN SLKTKNPKLK ILLSIGGYLF GSKGFHPMVD SSTSRLEFIN SIILFLRNHN FDGLDVSWIY PDQKENTHFT VLIHELAEAF QKDFTKSTKE RLLLTAGVSA GRQMIDNSYQ VEKLAKDLDF INLLSFDFHG SWEKPLITGH NSPLSKGWQD RGPSSYYNVE YAV

Protein concentration

CMV Pp28 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.

Product Description

Immunoreactive with sera of CMV-infected individuals.