| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Formulation |
CCDC90B protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
CCDC90B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (43-230 a.a) and having a molecular mass of 24.1kDa.CCDC90B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Coiled-Coil Domain Containing 90B (CCDC90B) is a member of the CCDC90 family. The CCDC90B protein is distinguished by the presence of a domain of unknown function, DUF1640. The DUF1640 domain is a characteristic of the entire protein with excluding of the first 23 amino acid residues which are a mitochondrial targeting site and the cleaved CCDC90B is expected to be a mitochondrial protein with no transmembrane spans or segments. CCDC90B is predicted to contain at least 3 specific phospohorylation sites. |
| Expression host |
E.coli. |
| Synonyms |
MDS011, MDS025, CUA003, Coiled-coil domain-containing protein 90B mitochondrial, CCDC90B. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSGYDRRPV DITPLEQRKL TFDTHALVQD LETHGFDKTQ AETIVSALTA LSNVSLDTIY KEMVTQAQQE ITVQQLMAHL DAIRKDMVIL EKSEFANLRA ENEKMKIELD QVKQQLMHET SRIRADNKLD INLERSRVTD MFTDQEKQLM ETTTEFTKKD TQTKSIISET SNKIDAEIAS LKTLMESNKL E. |
| Antibody Properties |
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Available Conjugates |
Blocking, staining and activating antibody. For staining, use 10ul/1,000,000 cells. Titer for blocking and activating T cells should be determined by the investigator. |
| Immunological function |
This antibody is also available conjugated to FITC. |
