CALB2 Antibody

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Clone

PAT5C5AT.

Applications

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Protein Background

Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to t

Synonyms

Calretinin, CR, Calb2, calbindin 2.

Reagent Appearance

Sterile filtered colorless solution.

Type

Mouse Anti Human Monoclonal.

Immunogen

Anti-human CALB2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CALB2 protein 1-271 amino acids purified from E. coli.

Ig Subclass

Mouse IgG1 heavy chain and k light chain.

Purification Method

CALB2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Protein formulation

1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.

Storage Procedures

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

Stability / Shelf Life

12 months at -20°C. 1 month at 4°C.

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Staining and blocking antibody. For staining, use 10ul/1,000,000 cells. This antibody will block signaling through the CD11a molecule. Titer for blocking should be determined by the investigator.

Immunological function

This antibody is only available conjugated to FITC.