ATP sulfurylase Yeast

Data sheet

Formulation

The ATP sulphurylase protein was lyophilized after dialysis against lyophilized from 10mM NaP buffer, 100mM NaCl, 10mM Lactose, 1% PEG pH 7.5 and 0.75mM DTT.

Solubility

Spin vial before opening. Reconstitute ATP sulphurylase with 5mM NaP pH-7.5 & 0.75mM DTT at a concentration ranging from 0.1mg 1mg per ml. Can be diluted further into other aqueous buffers. pH range between 7.0 8.5 is best.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containg 511 amino acids and having a Mw of 57.7 kDa. Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of s

Protein Background

ATP sulphurylase synthesizes adenosine 5-sul-phatophosphate from ATP and inorganic SO42-. This is the first reaction of a two step sequence in the formation of active sulphate, adenosine 3-phosphate5-sulphatophosphate, which is a sulphate donor for a wide

Expression host

Escherichia Coli.

Synonyms

Sulfate adenylyltransferase, EC 2.7.7.4, Sulfate adenylate transferase, SAT, ATP-sulfurylase, Methionine-requiring protein 3, ATPS.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Sulfate adenylate transferase although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ATPS should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended

Amino acid sequence

MPAPHGGILQ DLIARDALKK NELLSEAQSS DILVWNLTPR QLCDIELILN GGFSPLTGFL NENDYSSVVT DSRLADGTLW TIPITLDVDE AFANQIKPDT RIALFQDDEI PIAILTVQDV YKPNKTIEAE KVFRGDPEHP AISYLFNVAG DYYVGGSLEA IQLPQHYDYP GLRKTPAQLR LEFQSRQWDR VVAFQTRNPM HRAHRELTVR AAREANAKVL IHPVVGLTKP GD

Unit Definition

One unit produces 1.0 umole of ATP from APS and inorganic phosphate per min. at pH 8.0 and 30°C.

Biological Activity

12 Units/mg.