| Formulation |
ACTR3 protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
ACTR3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 441 amino acids (1-418 a.a) and having a molecular mass of 49.8kDa. ACTR3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
ARP3 Actin-Related Protein 3, also known as ACTR3, belongs to the actin family. The specific function of ACTR3 has not been established yet. On the other hand, ACTR3 is identified as being a major constituent of the ARP2/3 complex. In addition, this complex is located at the cell surface and vital to cell shape & motility through lamellipodial actin assembly and protrusion. 3 transcript variants encoding 2 different isoforms have been discovered for ACTR3. |
| Expression host |
Escherichia Coli. |
| Synonyms |
ARP3 Actin-Related Protein 3 Homolog (Yeast), ARP3, Actin-Like Protein 3, ARP3 (Actin-Related Protein 3, Yeast) Homolog, Actin-Related Protein 3. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAGRLPA CVVDCGTGYT KLGYAGNTEP QFIIPSCIAI KESAKVGDQA QRRVMKGVDD LDFFIGDEAI EKPTYATKWP IRHGIVEDWD LMERFMEQVI FKYLRAEPED HYFLLTEPPL NTPENREYTA EIMFESFNVP GLYIAVQAVL ALAASWTSRQ VGERTLTGTV IDSGDGVTHV IPVAEGYVIG SCIKHIPIAG RDITYFIQQL LRDREVGIPP EQSLETAKAV KERYSYVCPD LVKEFNKYDT DGSKWIKQYT GINAISKKEF SIDVGYERFL GPEIFFHPEF ANPDFTQPIS EVVDEVIQNC PIDVRRPLYK NIVLSGGSTM FRDFGRRLQR DLKRTVDARL KLSEELSGGR LKPKPIDVQV ITHHMQRYAV WFGGSMLAST PEFYQVCHTK KDYEEIGPSI CRHNPVFGVM S. |
