Carboxypeptidase-B Rat Recombinant ( Carboxypeptidase B Rat )

Data sheet

Formulation

The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.

Solubility

It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 35kDa is purified by standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carbox

Protein Background

Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kda, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine,

Expression host

Escherichia Coli.

Synonyms

Carboxypeptidase B, Cpb1, Cpb.

Reagent Appearance

Sterile Filtered lyophilized powder.

Stability

Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Unit Definition

One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.