Lipase-A Recombinant ( Lipase A )

Data sheet

Formulation

The protein was lyophilized without additives.

Solubility

It is recommended to reconstitute the lyophilized Lipase-A in sterile 10%-50% DMSO, isopropyl ether, petroleum ether, ethanol, acetone and isopropanol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques.

Protein Background

Lipase (EC 3.1.1.3) is a ubiquitous enzyme that catalyzes the hydrolysis of fats and oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester

Expression host

Escherichia Coli.

Reagent Appearance

Sterile Filtered lyophilized powder.

Stability

Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Unit Definition

Lipase-A was assayed using p-nitrophenyl acetate ( pNPA) as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 mol of p-nitrophenol per minute.

Biological Activity

580 units/mg powder.