Malate Dehydrogenase Chicken Recombinant ( MDH1 Chicken )

Data sheet

Formulation

Each mg of protein contains 0.59mg NaPO4.

Solubility

It is recommended to reconstitute the lyophilized Malate dehydrogenase in sterile 18M-cm H2O.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart.The MDH1 is purified by proprietary chromatographic techniques.

Protein Background

Malate dehydrogenase (EC1.1.1.37) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is not to be confused with malic enzyme, wh

Expression host

Escherichia Coli.

Synonyms

Malate dehydrogenase cytoplasmic, EC 1.1.1.37, Cytosolic malate dehydrogenase, MDHA, MOR2, MDH-s, MGC:1375, MDH1.

Reagent Appearance

Sterile lyophilized powder.

Stability

Lyophilized Malate dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MDH1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Unit Definition

One unit is defined as 1 umol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).

Specific Activity

Specific Activity Greater than 710U/mg protein.