View larger

Cynomolgus CD200R ELISA Kit

NS-E10003

$576.00

Data sheet

Assay Range 62.5 - 4000 pg/ml
Sensitivity 30 pg/ml.
Size 96T.
Storage Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C.
Expiration Six months at 2 - 8 ⁰C.
Application For quantitative determination of Cynomolgus CD200R.
Assay principle This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA).
Sample type Culture Supernates, Serum, plasma, body fluids, tissue lysates.

More info

 

Sample Volume
100 µL final volume, dilution factor varies on samples
 
Detection Method
Chromogenic 
 
 
Kit Components
 
 
 
1. Recombinant Cynomolgus CD200R standard: 40ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-Cynomolgus CD200R antibody.
 
 
3. Sample diluent buffer: 12 ml
 
 
4. HRP conjugated anti-Cynomolgus CD200R antibody:1 vial, dilution 1:500.
 
 
5. Detection Antibody diluention buffer: 12ml.
 
 
6. Assay diluent buffer: 12 ml.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.
 
 
 
 
Background
 

Cynomolgus CD200R is a member of the CD200R family.The CD200R family consists of one inhibitory member, CD200R and various numbers of activating genes.CD200 (OX2) is a broadly distributed cell surface glycoprotein that interacts with a structurally related receptor (CD200R) and is involved in regulation of macrophage function. CD200R is expressed in granulocytes, monocytes, most T-cells, neutrophils, basophils and a subset of NK, NKT and B-cells. CD200R limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. CD200R also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. 

   
Standard Curve

 

Download

© 2024 Novateinbio.com